Immobilization of Lipase from Candida rugosa on Chitosan Beads for Transesterification Reaction
نویسندگان
چکیده
منابع مشابه
Immobilization of Candida rugosa lipase on MCM-41 for the transesterification of cotton seed oil.
Present study demonstrated the preparation of MCM-41 as a support for the immobilization of Candida rugosa lipase by the physical adsorption technique. The lipase immobilized MCM-41 has been characterized by scanning electron microscopic and FTIR techniques. At pH 6, maximum lipase immobilization (250 mg/g) on MCM support has been observed and the immobilized lipase was employed as biocatalyst ...
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Lipase from Candida rugosa was immobilized on three different supports, i.e. Amberlite XAD7, poly(methylmethacrylate) (PMMA) and celite. With the conditions tested, maximum adsorption can be achieved after 30 min. The activities of the immobilized lipases were determined by the esterification reaction of oleic acid and butanol. The immobilized lipases were found to be very effective in the este...
متن کاملEffects of preparative parameters on the properties of chitosan hydrogel beads containing Candida rugosa lipase.
The influences of the pH, tripolyphosphate (TPP) concentration, and ionic strength of the gelling medium on the entrapment efficiency, release, and activity of lipase in chitosan hydrogel beads were studied. A solution of Candida rugosa lipase was prepared in a 1.5% w/v chitosan and 1% (v/v) acetic acid medium, and dropped into a TPP solution. Release of lipase in pH 7.2 Tris buffer was monitor...
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Magnetic silica aerogel in hydrophobic and hydrophilic forms were used as support to immobilize Candida rugosa lipase by adsorption method. Response surface methodology (RSM) was employed to study the effects of the three most important immobilization parameters, namely enzyme/support ratio (0.3-0.5, w/w), immobilization time (60-120 min) and alcohol percentage (20-40, %v/v) on the specific act...
متن کاملImmobilization of Lipase on Chitosan Beads for Removal of Pitch Particles from Whitewater during Papermaking
Pitch deposits originating from alkaline peroxide bleaching of mechanical pulps can seriously decrease the runnability of the paper machine when efforts have been made to increase the reuse of process water. In order to degrade pitch particles present in whitewater, lipase was immobilized on chitosan beads using a binary method. The operational stability of the immobilized lipase and its effica...
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ژورنال
عنوان ژورنال: American Journal of Applied Sciences
سال: 2009
ISSN: 1546-9239
DOI: 10.3844/ajassp.2009.1653.1657